Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p263 | (1) | WCRB2014

Comparison between egg yolk-based and coconut water-based extenders to freeze buffalo semen

Zorzetto Mariana , Sancler-Silva Yame , Dell'aqua Camila Freitas , Neto Carlos Ramires , Zocca Saulo , Ramos Alcides , Oba Eunice , Derussi Ana Augusta Pagnano

The aim of this experiment was compare two egg yolk-based (Tris and botu-Bov) and a coconut water-based (ACP-111) extenders to freeze buffalo semen. Four ejaculates of four buffalo bull were collected. Semen was divided in three groups: EY1 group, the semen was diluted with Tris-Yolk extender; EY2 group, the semen was diluted with Botu-Bov TM; CW group, the semen was diluted with ACP-111TM. The samples were packed in 0.5 ml straws containing 30×10<su...

ra0001p350 | (1) | WCRB2014

Effects of cooling and cryopreservation on sperm mitochondrial membrane and sperm motility

Sicherle Carmen Cecilia , de Paula Freitas-Dell'Aqua Camila , Ackermann Camila Louise , Derussi Ana Augusta Pagnano , Mothe Gabriele , de Souza Fabiana Ferreira , Papa Frederico O , Lopes Maria Denise

The aim of this study was to analyze the effects of refrigeration and cryopreservation on dog sperm special on sperm motility and mitochondrial membrane potential (MMP). A total of 15 ejaculates, first and second fractions, were collected from five dogs. Semen was diluted (80×106 sptz/ml) on Tris-egg-yolk medium with 8% of glycerol (one step), filled into 0.5 ml French straw and refrigerated at 5 °C for 1 h. After straws were suspended 6 cm above liquid ni...

ra0001p296 | (1) | WCRB2014

Evaluation of protein profile in uterine secretion on early cyclic and gestational diestrus in bitches

Derussi Ana Augusta Pagnano , Volpato Rodrigo , de Freitas Guaitolini Carlos Renato , Ackermann Camila Louise , Taffarel Marilda Onghero , Cardoso Guilherme Schiess , Villaverde Ana Izabel Silva Balbin , de Souza Fabiana Ferreira , Lopes Maria Denise

Introduction: During peri-implantation period the canines conceptus are nourished by secretions in uterine lumen, a phenomenon known as histotrophic nutrition, in which several proteins are present. The aim of this study was to compare the protein profile in uterine secretion in early pregnancy and early diestrus cyclic using two-dimensional electrophoresis technique.Material and methods: For that, ten bitches were submitted to OHE on the 12th day after ...